Coding

Part:BBa_J09271:Design

Designed by: Jon Badalamenti   Group: iGEM2005   (2006-10-26)


motB flagellar protein, without endogenous RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 310


Design Notes

We had to design this part to address leaky expression from R0062. In order to induce motility with HSL, we must be able to demonstrate both repression and induction. S03271 contains the endogenous RBS of motB and is thought to be strong. Leaky expression could be diminished by adding weaker ribosome binding sites upstream of this part.


Source

This part was amplified by PCR from the genome of E. coli strain RP437 (wild-type for motility)

References